A total of 48 Siboney de Cuba cattle blood samples (35 females and 13 males, with age between 24 and 30 months) were collected. The DNA was extracted from blood using phenol-chloroform standard protocol. Animals were chosen on the basis of the viability in the experimental herd of the Centro Nacional de Sanidad Agropecuaria (CENSA, Cuba).
All animals were genotyped with the GGP Bovine 100k BeadChip (Bos taurus x Bos indicus) containing approximately 100,000 SNPs (Illumina, San Diego, CA, USA). The genomic coordinates for each marker were obtained using the assembled cow genome (ARS-UCD 1.2). SNPs assigned to sexual chromosomes or without position were discarded. The software PLINK v. 1.9 (Chang et al., 2015) was used to perform filtering and quality control on the dataset, using the following criteria: i) minor allele frequency (MAF) ≥ 0.05; ii) genotype call rate for a SNP ≥ 0.95; and iii) individual call rate ≥ 0.90. After quality check, 48 animals and 83,314 SNPs were retained.

The data was subject to restrictions due to a formal agreement between the University of Padova and the Centro Nacional de Sanidad Agropecuaria de Cuba.