Data in "Photodamage_Data.zip"
For more details refer to the preprint in BioRxiv doi: https://doi.org/10.1101/2021.05.14.443726
Data was obtained by analyzing image sequences acquired with a 2-photon microscope and performing in vivo photodamage of a basal keratinocyte in mouse earlobe skin. After photodamage, Ca2+ waves propagated circularly from the photodamaged cell to the surraounding epidermal cells.
Pharmacolological interference experiments were performed and differences between control and drug microinjection were quantified.
The folder is composed of 9 subfolder, each containing 8 .ods files, each corresponding to a different experimental condition:
- Apyrase: microinjection of vehicle solution containing apyrase 500 U/ml;
- ARL 67156: microinjection of vehicle solution containing ARL 67156 400 µM;
- Carbenoxolone: microinjection of vehicle solution containing carbenoxolone 400 µM;
- EGTA: microinjection of vehicle solution containing EGTA 5 mM;
- PPADS: microinjection of vehicle solution containing PPADS 625 µM;
- Probenecid: microinjection of vehicle solution containing probenecid 4 mM;
- TAT-gap19: microinjection of vehicle solution containing TAT-gap19 200 µM;
- Thapsigargin: microinjection of vehicle solution containing thapsigargin 400 nM;
- Vehicle solution: microinjection of only vehicle solution;
Each subfolder contains results obtained in 2 different mice in control condition and after drug microinjection. The same str
Example:
FILES: - Mouse1_Drug_dF_F0.ods: relative change in fluorescence intensity as concequence of single keratinocyte photodamage after drug microinjection in mouse 1.
SHEET 1: Time
SHEET 2: Order 1: dF/F0 from ROIs distant 10 µm from photodamaged cell at increasing timesteps
SHEET 3: Order 2: dF/F0 from ROIs distant 20 µm from photodamaged cell at increasing timesteps
SHEET 4: Order 3: dF/F0 from ROIs distant 30 µm from photodamaged cell at increasing timesteps
SHEET 5: Order 4: dF/F0 from ROIs distant 40 µm from photodamaged cell at increasing timesteps
SHEET 6: Order 5: dF/F0 from ROIs distant 50 µm from photodamaged cell at increasing timesteps
SHEET 7: Order 6: dF/F0 from ROIs distant 60 µm from photodamaged cell at increasing timesteps
SHEET 8: Order 7: dF/F0 from ROIs distant 70 µm from photodamaged cell at increasing timesteps
SHEET 9: Order 8: dF/F0 from ROIs distant 80 µm from photodamaged cell at increasing timesteps
- Mouse1_Drug_Radius.ods relative change in fluorescence intensity as concequence of single keratinocyte photodamage after drug microinjection in mouse 1.
SHEET 1: Time
SHEET 2: Radius at increasing timestep in different waves after drug microinjection in mouse 1.
- Mouse1_Control_dF_F0.ods relative change in fluorescence intensity as concequence of single keratinocyte photodamage in control condition in mouse 1.
SHEET 1: Time
SHEET 2: Order 1: dF/F0 from ROIs distant 10 µm from photodamaged cell
SHEET 3: Order 2: dF/F0 from ROIs distant 20 µm from photodamaged cell
SHEET 4: Order 3: dF/F0 from ROIs distant 30 µm from photodamaged cell
SHEET 5: Order 4: dF/F0 from ROIs distant 40 µm from photodamaged cell
SHEET 6: Order 5: dF/F0 from ROIs distant 50 µm from photodamaged cell
SHEET 7: Order 6: dF/F0 from ROIs distant 60 µm from photodamaged cell
SHEET 8: Order 7: dF/F0 from ROIs distant 70 µm from photodamaged cell
SHEET 9: Order 8: dF/F0 from ROIs distant 80 µm from photodamaged cell
- Mouse1_Control_Radius.ods SHEET 1: Time
SHEET 2: Radius at increasing timestep in different waves in control condition in mouse 1.
- Mouse2_Drug_dF_F0.ods relative change in fluorescence intensity as concequence of single keratinocyte photodamage after drug microinjection in mouse 2.
SHEET 1: Time
SHEET 2: Order 1: dF/F0 from ROIs distant 10 µm from photodamaged cell
SHEET 3: Order 2: dF/F0 from ROIs distant 20 µm from photodamaged cell
SHEET 4: Order 3: dF/F0 from ROIs distant 30 µm from photodamaged cell
SHEET 5: Order 4: dF/F0 from ROIs distant 40 µm from photodamaged cell
SHEET 6: Order 5: dF/F0 from ROIs distant 50 µm from photodamaged cell
SHEET 7: Order 6: dF/F0 from ROIs distant 60 µm from photodamaged cell
SHEET 8: Order 7: dF/F0 from ROIs distant 70 µm from photodamaged cell
SHEET 9: Order 8: dF/F0 from ROIs distant 80 µm from photodamaged cell
- Mouse2_Drug_Radius.ods SHEET 1: Time
SHEET 2: Radius at increasing timestep in different waves after drug microinjection in mouse 2.
- Mouse2_Control_dF_F0.ods relative change in fluorescence intensity as concequence of single keratinocyte photodamage in control condition in mouse 2.
SHEET 1: Time
SHEET 2: Order 1: dF/F0 from ROIs distant 10 µm from photodamaged cell
SHEET 3: Order 2: dF/F0 from ROIs distant 20 µm from photodamaged cell
SHEET 4: Order 3: dF/F0 from ROIs distant 30 µm from photodamaged cell
SHEET 5: Order 4: dF/F0 from ROIs distant 40 µm from photodamaged cell
SHEET 6: Order 5: dF/F0 from ROIs distant 50 µm from photodamaged cell
SHEET 7: Order 6: dF/F0 from ROIs distant 60 µm from photodamaged cell
SHEET 8: Order 7: dF/F0 from ROIs distant 70 µm from photodamaged cell
SHEET 9: Order 8: dF/F0 from ROIs distant 80 µm from photodamaged cell
- Mouse2_Control_Radius.ods: SHEET 1: Time
SHEET 2: Radius at increasing timestep in different waves in control condition in mouse 2.