Occhi, Gianluca and Dalle Nogare, Mattia (2025) Transcriptomic profiling of GH-secreting pituitary neuroendocrine tumors for tissue deconvolution. [Data Collection]
Collection description
Ten GH-PitNET samples were collected from acromegalic patients treated surgically. Total RNA was extracted using the Quick-DNA/RNA kit (Zymo Research), then quality-checked with Agilent Bioanalyzer and NanoDrop. Libraries were prepared using KAPA RNA HyperPrep with RiboErase (Roche) and sequenced (150 bp paired-end) on an Illumina NovaSeq X Plus. Gene-level counts presented in the table were quantified using Salmon and summarized with tximport.
| DOI: |  10.25430/researchdata.cab.unipd.it.00001604 |
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| Keywords: | Bulk RNA-seq, Pituitary Neuroendocrine Tumor (PitNET), Deconvolution, Cellular heterogeneity | |||||||||
| Subjects: | Life Sciences > Genetics, 'Omics', Bioinformatics and Systems Biology: Molecular genetics, quantitative genetics, genetic epidemiology, epigenetics, genomics, metagenomics, glycomics, bioinformatics, computational biology, biostatistics, systems biology proteomics, metab > Bioinformatics Life Sciences > Genetics, 'Omics', Bioinformatics and Systems Biology: Molecular genetics, quantitative genetics, genetic epidemiology, epigenetics, genomics, metagenomics, glycomics, bioinformatics, computational biology, biostatistics, systems biology proteomics, metab > Transcriptomics |
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| Department: | Departments > Dipartimento di Biologia (DiBio) | |||||||||
| Depositing User: | Mattia Dalle Nogare | |||||||||
| Date Deposited: | 25 Jul 2025 08:42 | |||||||||
| Last Modified: | 25 Jul 2025 08:42 | |||||||||
| Creators/Authors: |
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| Type of data: | Text | |||||||||
| Contributors: |
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| Research funder: | MUR | |||||||||
| Research project title: | Epigenomic and Transcriptomic landscape of GH-secreting pituitary adenomas in a “paradoxical” background | |||||||||
| Grant number: | Prot. 2022HTYFEL | |||||||||
| Collection period: |
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| Data collection method: | Ten GH-secreting pituitary neuroendocrine tumor (GH-PitNET) samples were collected from acromegalic patients undergoing transsphenoidal surgery at Padova University Hospital. A portion of each tumor specimen was immediately preserved in RNAlater (Ambion), stored at 4 °C for 24 hours, and then transferred to −20 °C for long-term storage prior to RNA extraction. | |||||||||
| Statement on legal, ethical and access issues: | The study was conducted in accordance with ethical guidelines consistent with the Declaration of Helsinki and was approved by the Ethical Committee of Azienda Ospedaliera di Padova (approval no. AOP1782). | |||||||||
| Resource language: | English | |||||||||
| Metadata language: | English | |||||||||
| Publisher: | Research Data Unipd | |||||||||
| Date: | 25 July 2025 | |||||||||
| Copyright holders: | The Author | |||||||||
| URI: | https://researchdata.cab.unipd.it/id/eprint/1604 |
orcid.org/0000-0003-4065-4677
