University of Padua

EVs from hypoxic NB cells stimulate migration and invasion of target cells via miR-210-3p

Cimetta, Elisa (2022) EVs from hypoxic NB cells stimulate migration and invasion of target cells via miR-210-3p. [Data Collection]

Collection description

Tumor hypoxia stimulates release of extracellular vesicles (EVs) that facilitate short- and long-range intercellular communication and metastatization. Albeit hypoxia and EVs release are known features of Neuroblastoma (NB), a metastasis-prone childhood malignancy of the sympathetic nervous system, whether hypoxic EVs can facilitate NB dissemination is unclear. Here we show that EVs from hypoxic NB cells promote pro-metastasis features in vitro and in an in vivo model. EVs from NB cells cultured at different oxygen tensions did not differ for type and abundance of surface markers and for biophysical properties. However, EVs derived from hypoxic NB cells were more potent than their normoxic counterpart in inducing NB cells migration and colony formation. We characterized and compared the microRNA (miRNA) cargo of EVs from NB cells cultured at different oxygen tensions and found that miR-210-3p was the most upregulated. Mechanistically, we identified that the pro-metastatic features of EVs from hypoxic NB cells derived from a miR-210-3p enrichment in their cargo. Indeed, overexpression of miR-210-3p in normoxic EVs conferred them pro-metastatic features, whereas miR-210-3p silencing suppressed the metastatic ability of hypoxic EVs both in vitro and in in vivo zebrafish model. Our data identify a role for hypoxic EVs in the cellular and microenvironmental changes favoring NB dissemination.

DOI: 10.25430/
Keywords: Neuroblastoma, extracellular vesicles, miRNA, hypoxia, metastasis
Subjects: Life Sciences > Cellular and Developmental Biology: Cell biology, cell physiology, signal transduction, organogenesis, developmental genetics, pattern formation, stem cell biology, in plants and animals and where appropriate in microorganisms > Cell signalling and signal transduction
Life Sciences > Applied Life Sciences, Biotechnology and Molecular and Biosystems engineering: Applied plant and animal sciences; forestry; food sciences; applied biotechnology; environmental, and marine biotechnology; applied bioengineering; biomass, biofuels; biohazard > Applied biotechnology (including, transgenic organisms, applied genetics and genomics, biosensors, bioreactors, microbiology, bioactive compounds)
Department: Departments > Dipartimento di Ingegneria industriale (DII)
Depositing User: Elisa Cimetta
Date Deposited: 31 Aug 2022 15:55
Last Modified: 02 Sep 2022 10:30
Type of data: Mixed
Research funder: European Commission
Research project title: MICRONEX
Grant number: 759467 - UERI17
Collection period:
Resource language: English
Metadata language: English
Publisher: Research Data Unipd
Date: 31 August 2022
Copyright holders: The Author

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