Cimetta, Elisa
(2022)
A porous gelatin methacrylate-based material for 3D cell-laden constructs.
[Data Collection]
Collection description
All original data for the manuscript "A porous gelatin methacrylate-based material for 3D cell-laden constructs"
Abstract: Three-dimensional constructs are fundamental in tissue engineering and cancer modelling, generating a demand for tailored materials creating a suitable microenvironment for cell culture and amenable to be shaped through techniques such as bioprinting. Gelatin methacrylate (GelMA) is a well-known functionalized natural polymer with good printability and binding motifs allowing cell adhesion. Encapsulated cells however tend to retain a spherical shape because of the tight internal structure characterized by microscale pores. In this work, we blended GelMa with Pluronic F-127 (PLU) to modify the hydrogels’ internal porosity. We found that adding 0.5% w/v PLU resulted in a structure characterized by larger mesoscale pores surrounded by the typical microscale GelMA network. The increase in porosity also led to an increased swelling ability and a slight decrease in the Young’s modulus. All blends formed stable hydrogels both when cast in annular molds and bioprinted in more complex structures. Embedded tumor and non-tumor cells maintained high cell viability and behaved differently: neuroblastoma cells (SK-N-AS) showed a tendency to form aggregates inside the mesoscale pores, while mesenchymal stem cells (MSCs) stretched in all three dimensions, forming cell-cell and cell-ECM interactions using the free space of the large pores.
| DOI: |
 10.25430/researchdata.cab.unipd.it.00000688 |
| Keywords: |
Hydrogels; bioprinting; cell-laden constructs; cancer modeling. |
| Subjects: |
Physical:Sciences and Engineering > Products and Processes Engineering: Product design, process design and control, construction methods, civil engineering, energy processes, material engineering > Chemical engineering, technical chemistry |
| Department: |
Departments > Dipartimento di Ingegneria industriale (DII) |
| Depositing User: |
Elisa Cimetta
|
| Date Deposited: |
09 Aug 2022 06:29 |
| Last Modified: |
09 Aug 2022 06:29 |
| Creators/Authors: |
|
| Type of data: |
Image |
| Contributors: |
| Contribution | Name | Email |
|---|
| Author | Maggiotto, Federico | federico.maggiotto@studenti.unipd.it | | Author | Bova, Lorenzo | lnbvnano@gmail.com |
|
| Research funder: |
European Commission |
| Research project title: |
MICRONEX |
| Grant number: |
UERI17 |
| Collection period: |
|
| Resource language: |
English |
| Metadata language: |
English |
| Publisher: |
Research Data Unipd |
| Date: |
8 August 2022 |
| Copyright holders: |
The Author |
| URI: |
https://researchdata.cab.unipd.it/id/eprint/688 |
| Creators/Authors: |
|
| Type of data: |
Image |
| Contributors: |
| Contribution | Name | Email |
|---|
| Author | Maggiotto, Federico | federico.maggiotto@studenti.unipd.it | | Author | Bova, Lorenzo | lnbvnano@gmail.com |
|
| Research funder: |
European Commission |
| Research project title: |
MICRONEX |
| Grant number: |
UERI17 |
| Collection period: |
|
| Resource language: |
English |
| Metadata language: |
English |
| Publisher: |
Research Data Unipd |
| Date: |
8 August 2022 |
| Copyright holders: |
The Author |
| Last Modified: |
09 Aug 2022 06:29 |
|
orcid.org/0000-0002-8632-3999
